PathHunter® eXpress NMBR Total GPCR Internalization Assay

Background Information:

The PathHunter® NMBR Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. neuromedin B) based internalization of NMBR via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged NMBR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the NMBR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Bombesin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

BB1

Protein Aliases:

bombesin receptor 1

Accession Number:

NM_002511.2

UniProt Number:

P28336

Gene Name:

NMBR

Gene ID:

4829

Gene Aliases:

BB1|BB1R

Target Species:

Human

Cell Background:

U2OS