Biological Information
Background Information:
The PathHunter® mCNR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of mCNR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged mCNR1 and EA-tagged β-Arrestin-2. Activation of mCNR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein.
Target Class:
GPCR
Family:
Cannabinoid
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
CB1
Protein Aliases:
CB1|CANN6
Accession Number:
NM_007726
UniProt Number:
P47746
Gene Name:
CNR1
Gene ID:
12801
Gene Aliases:
CB1K5|CB-R|CB1|CANN6|CB1A
Target Species:
Mouse
Cell Background:
CHO-K1
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
CNS/Addiction/Pain
Additional Information
Brand:
PathHunter®