Biological Information

Background Information:

The PathHunter® mCNR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of mCNR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged mCNR1 and EA-tagged β-Arrestin-2. Activation of mCNR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein.

Target Class:

GPCR

Family:

Cannabinoid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CB1

Protein Aliases:

CB1|CANN6

Accession Number:

NM_007726

UniProt Number:

P47746

Gene Name:

CNR1

Gene ID:

12801

Gene Aliases:

CB1K5|CB-R|CB1|CANN6|CB1A

Target Species:

Mouse

Cell Background:

CHO-K1

Usage

Product Type:

eXpress Assay Kits

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

CNS/Addiction/Pain

Additional Information

Brand:

PathHunter®