PathHunter® eXpress MCHR1 U2OS β-Arrestin GPCR Assay

Background Information:

The PathHunter® MCHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MCH) based activation of MCHR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged MCHR1 and EA-tagged β-Arrestin-2. Activation of MCHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Melanin-Concentrating Hormone

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

MCH1

Protein Aliases:

MCH-R1|MCHR-1|G-protein coupled receptor 24|MCH-1R|MCH1R|MCHR|SLC-1|Somatostatin receptor-like protein

Accession Number:

AY562945

UniProt Number:

Q99705

Gene Name:

MCHR1

Gene ID:

2847

Gene Aliases:

SLC1|MCH1R

Target Species:

Human

Cell Background:

U2OS