Biological Information
Background Information:
The PathHunter® mCCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of mCCR7 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged mCCR7 and EA-tagged β-Arrestin-2. Activation of mCCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein.
Target Class:
GPCR
Family:
Chemokine
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
CCR7
Protein Aliases:
CC-CKR-7|CCR-7|BLR2|CDw197|Epstein-Barr virus-induced G-protein coupled receptor 1|EBI1|EBV-induced G-protein coupled receptor 1|MIP-3 beta receptor|CD antigen CD197
Accession Number:
NM_007719.2
UniProt Number:
P47774
Gene Name:
CCR7
Gene ID:
12775
Gene Aliases:
BLR2|CDw197|CD197
Target Species:
Mouse
Cell Background:
CHO-K1
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®