PathHunter® eXpress Flt3 Activity Assay

Background Information:

The PathHunter® Flt3 Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activation via detection of resulting Flt3 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress a PK-tagged engineered fusion of the catalytic domain of Flt3 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

FLT3

Protein Aliases:

FL cytokine receptor|Fetal liver kinase-2|FLK-2|Fms-like tyrosine kinase 3|FLT-3|Stem cell tyrosine kinase 1|STK-1|CD antigen CD135

Accession Number:

NM_005228.3

UniProt Number:

P36888

Gene Name:

FLT3

Gene ID:

2322

Gene Aliases:

STK1|FLK2|CD135

Target Species:

Human

Cell Background:

U2OS