Biological Information
Background Information:
The PathHunter® GPR120 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. free fatty acids) based activation of GPR120 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged GPR120 and EA-tagged β-Arrestin-2. Activation of GPR120 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Free Fatty Acid
Sub Family:
Class A
Protein Name:
FFA4 (GPR120)
Protein Aliases:
G-protein coupled receptor 129|G-protein coupled receptor GT01|G-protein coupled receptor PGR4|Omega-3 fatty acid receptor 1
Accession Number:
NM_001287319
UniProt Number:
C8YUV0
Gene Name:
FFAR4
Gene ID:
102136156
Gene Aliases:
PGR4
Target Species:
Cynomolgus Monkey
Cell Background:
CHO-K1
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®