PathHunter® eXpress CXCR7 Activated GPCR Internalization Assay

Background Information:

The PathHunter® CXCR7 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CXCL12/SDF-1α) based internalization of CXCR7 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR7. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

ACKR3 (CXCR7)

Protein Aliases:

CXC-R7|CXCR-7|Chemokine orphan receptor 1|G-protein coupled receptor 159|G-protein coupled receptor RDC1 homolog|RDC-1

Accession Number:

NM_020311.2

UniProt Number:

P25106

Gene Name:

ACKR3

Gene ID:

57007

Gene Aliases:

CMKOR1|CXCR7|GPR159|RDC1

Target Species:

Human

Cell Background:

U2OS