PathHunter® eXpress CXCR3 Activated GPCR Internalization Assay

Background Information:

The PathHunter® CXCR3 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CXCL11/I-TAC) based internalization of CXCR3 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR3. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR3-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR3

Protein Aliases:

CXCR-3|CKR-L2|G protein-coupled receptor 9|Interferon-inducible protein 10 receptor|IP-10 receptor|CD antigen CD183

Accession Number:

AY242128

UniProt Number:

P49682

Gene Name:

CXCR3

Gene ID:

2833

Gene Aliases:

CKR-L2|CMKAR3|IP10-R|MigR|CD183

Target Species:

Human

Cell Background:

U2OS