PathHunter® eXpress CXCR2 Activated GPCR Internalization Assay

Background Information:

The PathHunter® CXCR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CXCL8/IL-8) based internalization of CXCR2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR2 (IL-8RB)

Protein Aliases:

CXCR-2|CDw128b|GRO/MGSA receptor|High affinity interleukin-8 receptor B|IL-8R B|IL-8 receptor type 2|CD antigen CD182

Accession Number:

NM_001557

UniProt Number:

P25025

Gene Name:

CXCR2

Gene ID:

3579

Gene Aliases:

CMKAR2|CD182

Target Species:

Human

Cell Background:

U2OS