Biological Information
Background Information:
The PathHunter® eXpress c-KIT Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-KIT dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged c-KIT and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Kinase
Family:
RTK
Sub Family:
Protein Tyrosine
Protein Name:
Kit
Protein Aliases:
Piebald trait protein|PBT|Proto-oncogene c-Kit|Tyrosine-protein kinase Kit|p145 c-kit|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog|CD antigen CD117
Accession Number:
NM_000222.2
UniProt Number:
P10721
Gene Name:
KIT
Gene ID:
3815
Gene Aliases:
CD117|SCFR|C-Kit
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
Assay Measures:
Functional Activity
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
PathHunter®