PathHunter® eXpress β-Catenin Nuclear Translocation Assay

Background Information:

The PathHunter® eXpress β-Catenin Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the Wnt-FzGSK3β pathway. This kit assesses ligand (e.g. Wnt 3a) based pathway activation via detection of resulting β-Catenin nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged β-Catenin and nuclear localized EA. Upon pathway activation, induced nuclear translocation of β-Catenin leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Nuclear Protein

Protein Name:

beta-Catenin

Accession Number:

NM_001904

UniProt Number:

P35222

Gene Name:

CTNNB1

Gene ID:

1499

Gene Aliases:

CTNNB|OK/SW-cl.35|PRO2286

Target Species:

Human

Cell Background:

U2OS