PathHunter® CHO-K1 C5AR1 Bioassay Kit

Background Information:

The PathHunter® CHO-K1 C5AR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Complement Peptide

Sub Family:

Class A

Protein Name:

C5aR

Protein Aliases:

C5a-R|C5aR|CD antigen CD88

Accession Number:

NM_001736

UniProt Number:

P21730

Gene Name:

C5AR1

Gene ID:

728

Gene Aliases:

C5A|C5AR|CD88

Target Species:

Human

Cell Background:

CHO-K1