Biological Information

Background Information:

The PathHunter® β-Arrestin2 Retroparticles are MMLV particles designed to transduce cells of interest with β-Arrestin2 fused to EA. In conjunction with a plasmid expressing a PK-tagged GPCR of interest, this allows for these cells to be used for β-Arrestin2 recruitment assays. This product uses EFC technology (https://www.discoverx.com/technologies-platforms/enzyme-fragment-complementation-technology). Ligand induced recruitment of β-Arrestin2 to the activated GPCR leads to complementation and produces EFC signal. These retroparticles ship as 4 vials of cryopreserved retroparticles.

Target Class:

GPCR

Usage

Product Type:

Retroparticles

Application:

Drug Discovery & Development

Storage Conditions:

Short term (24 h): Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®