PathHunter® AGTRL1 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® AGTRL1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. apelin-13) based internalization of AGTRL1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Apelin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

APJ

Protein Aliases:

APJ (apelin) receptor

Accession Number:

NM_005161

UniProt Number:

P35414

Gene Name:

APLNR

Gene ID:

187

Gene Aliases:

FLJ90771|APJ|APJR

Target Species:

Human

Cell Background:

U2OS