PathHunter® Aflibercept Bioassay Kit

Background Information:

The PathHunter® Aflibercept Bioassay kit provides an easy-to-use cell based assay to determine Aflibercept potency and detect neutralizing antibodies. This bioassay assesses VEGFA-induced dimerization of VEGFR2 receptors. This product uses EFC technology. The included cells overexpress VEGFR2 target protein fused to PK alongside VEGFR2 target protein fused to EA. Upon ligand-induced dimerization of VEGFR2, detectable EFC signal is produced. This bioassay has been optimized and qualified with the marketed biologic drug, Eylea® which is not provided in the bioassay kit. Eyelea® is a registered trademark of Regeneron Pharmaceutical, Inc. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

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Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

KDR (VEGFR2)

Protein Aliases:

vascular endothelial growth factor receptor 2|fetal liver kinase 1

Accession Number:

NM_002253.2

UniProt Number:

P35968

Gene Name:

KDR

Gene ID:

3791

Gene Aliases:

FLK1|VEGFR|VEGFR2|CD309

Target Species:

Human

Cell Background:

HEK293