InCELL Hunter™ Human CSNK1D Kinase Target Engagement Cell-Based Assay Kit (A549)

Background Information:

The InCELL Hunter™ Human CSNK1D Kinase Target Engagement Cell-Based Assay Kit (A549) utilizes the InCELL Hunter™ A549 CSNK1D Kinase Binding cell line. The stable engineered cell line is intended for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of CSNK1D via detection of CSNK1D levels. This product uses EFC technology. The cell line included overexpresses CSNK1D's kinase domain fused to PL. In the absence of a compound that binds and stabilizes the target, this protein fragment is naturally degraded and no EFC signal is produced. When the compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

CK1

Sub Family:

Protein Ser/Thr

Protein Name:

CK1delta

Protein Aliases:

CKId|Tau-protein kinase CSNK1D

Accession Number:

NC_000017.11

UniProt Number:

P48730

Gene Name:

CSNK1D

Gene ID:

1453

Gene Aliases:

HCKID|CKID|CKIdelta

Target Species:

Human

Cell Background:

A549