InCELL Hunter™ eXpress PIM1 Kinase Binding Assay

Background Information:

The InCELL Hunter™ eXpress PIM1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of PIM1 via detection of PIM1 levels. This product uses EFC technology. The cells included overexpress PIM1 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Kinase

Family:

CAMK

Sub Family:

Protein Ser/Thr

Protein Name:

Pim1

Accession Number:

NC_000006.12

UniProt Number:

P11309

Gene Name:

PIM1

Gene ID:

5292

Target Species:

Human

Cell Background:

A549