InCELL Hunter™ eXpress BTK Kinase Binding Assay

Background Information:

The InCELL Hunter™ eXpress BTK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BTK via detection of BTK levels. This product uses EFC technology. The cells included overexpress BTK target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Kinase

Family:

TK

Sub Family:

Protein Tyrosine

Protein Name:

BTK

Protein Aliases:

Bruton's tyrosine kinase

Accession Number:

NC_000023.11

UniProt Number:

Q06187

Gene Name:

BTK

Gene ID:

695

Gene Aliases:

ATK|XLA|PSCTK1

Target Species:

Human

Cell Background:

A549