Biological Information

Background Information:

The InCELL Hunter™ eXpress A549 GLP Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of GLP via detection of GLP levels. This product uses EFC technology. The cells included overexpress GLP target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Epigenetic

Family:

Histone Methyltransferase (HMT)

Sub Family:

Writer

Protein Name:

EHMT1

Protein Aliases:

GLP

Accession Number:

NM_024757

UniProt Number:

Q9H9B1

Gene Name:

EHMT1

Gene ID:

79813

Gene Aliases:

Eu-HMTase1|FLJ12879|KIAA1876|bA188C12.1|KMT1D

Target Species:

Human

Cell Background:

A549

Usage

Product Type:

eXpress Assay Kits

Application:

Target Engagement

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover

Assay Information

Assay Type:

Cell Based Binding

Assay Measures:

Target Engagement

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Additional Information

Brand:

InCELL Hunter™