Biological Information
Background Information:
The InCELL Hunter™ eXpress A549 GLP Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of GLP via detection of GLP levels. This product uses EFC technology. The cells included overexpress GLP target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Epigenetic
Family:
Histone Methyltransferase (HMT)
Sub Family:
Writer
Protein Name:
EHMT1
Protein Aliases:
GLP
Accession Number:
NM_024757
UniProt Number:
Q9H9B1
Gene Name:
EHMT1
Gene ID:
79813
Gene Aliases:
Eu-HMTase1|FLJ12879|KIAA1876|bA188C12.1|KMT1D
Target Species:
Human
Cell Background:
A549
Usage
Product Type:
eXpress Assay Kits
Application:
Target Engagement
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based Binding
Assay Measures:
Target Engagement
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
InCELL Hunter™