Biological Information
Background Information:
The ChemiSCREEN™ VPAC1 Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. VIP) based activation of VIPR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses VIPR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of VIPR1 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
VIP and PACAP
Sub Family:
Class B
Protein Name:
VIP1 (VPAC1)
Protein Aliases:
PVR2|HVR1|RDC1|VIP and PACAP receptor 1|PACAP-R2|pituitary adenylate cyclase-activating polypeptide type II receptor|VIP-R1
Accession Number:
NM_004624
UniProt Number:
P32241
Gene Name:
VIPR1
Gene ID:
7433
Gene Aliases:
VPAC1|RDC1|HVR1|VPAC1R
Target Species:
Human
Cell Background:
Chem-1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiSCREEN™