ChemiSCREEN™ GPR109A Nicotinic Acid Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ GPR109A Nicotinic Acid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. nicotinic acid) based activation of HCAR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HCAR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HCAR2 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Hydroxycarboxylic Acid

Sub Family:

Class A

Protein Name:

GPR109A

Protein Aliases:

niacin receptor 1|HCA2

Accession Number:

NM_177551.3

UniProt Number:

Q8TDS4

Gene Name:

HCAR2

Gene ID:

338442

Gene Aliases:

HCA2|HM74A|PUMAG|Puma-g|NIACR1

Target Species:

Human

Cell Background:

Chem-4