ChemiBRITE EP4 Prostanoid Receptor Stable Cell Line

Background Information:

The ChemiBRITE EP4 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER4 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER4 , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

EP4

Protein Aliases:

PGE2 receptor EP4 subtype|Prostanoid EP4 receptor

Accession Number:

AY429109

UniProt Number:

P35408

Gene Name:

PTGER4

Gene ID:

5734

Gene Aliases:

EP4|PTGER2

Target Species:

Human

Cell Background:

HEK293