ChemiBRITE EP1 Prostanoid Receptor Stable Cell Line

Background Information:

The ChemiBRITE EP1 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 , a naturally Gq coupled GPCR. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER1 , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

EP1

Protein Aliases:

PGE2 receptor EP1 subtype|Prostanoid EP1 receptor

Accession Number:

NM_000955.2

UniProt Number:

P34995

Gene Name:

PTGER1

Gene ID:

5731

Gene Aliases:

EP1

Target Species:

Human

Cell Background:

HEK293