cAMP Hunter™ Human GLP-1 (GLP1R) Gs Stable Cell Line Assay (CHO-K1)
The cAMP Hunter™ Human GLP-1 (GLP1R) Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GLP1R (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell line vials, all required culture reagents for maintaining the cells in continuous culture, and detection reagents for running the assay.
Catalog #: 795-0062C2
Size: Kit
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Biological Background
GLP1R (glucagon-like peptide-1 receptor) is a class B GPCR that plays a central role in metabolic regulation by mediating cAMP signaling in response to GLP-1 and related agonists. It is an important target in metabolic diseases, especially type 2 diabetes and obesity, where GLP-1-based therapies have become highly effective treatment options and continue to drive drug discovery interest.
The cAMP Hunter™ Human GLP-1 (GLP1R) Gs Stable Cell Line Assay (CHO-K1) enables quantitative measurement of receptor activation through intracellular cAMP detection, supporting potency, efficacy, and rank-order profiling of small molecules and biologics. Its stable CHO-K1 format, cryopreserved cells, and chemiluminescent EFC readout make it a practical and robust tool for GPCR pharmacology and mechanism-of-action studies.
Product Highlights
- Stable CHO-K1 cell line overexpressing wild-type human GLP1R for functional GPCR studies.
- Measures receptor activation by quantifying intracellular cAMP with sensitive chemiluminescent EFC detection.
- Supports potency, efficacy, and rank-order profiling of small molecules and biologics.
- Includes cryopreserved cells plus key cell culture and detection reagents for assay setup.
- Designed for drug discovery and development workflows in metabolic disease research.
cAMP Hunter Human GLP1R Gs Stable Cell Line Assay (CHO-K1) Assay Principle
In the cAMP Hunter Human GLP 1 (GLP1R) Gs Stable Cell Line Assay (CHO K1), CHO K1 cells overexpressing human GLP 1R leverage the receptor’s natural Gαs coupling to monitor activation. When stimulated by GLP 1 or related agonists, GLP1R activates adenylyl cyclase, which catalyzes ATP conversion to 3′ 5′ cyclic adenosine monophosphate (cAMP). The resulting increase in intracellular cAMP is quantified using a homogeneous, gain of signal competitive immunoassay based on Enzyme Fragment Complementation (EFC) technology. Signal intensity correlates directly with cellular cAMP levels, such that greater GLP 1R activation produces higher cAMP concentrations and larger assay signals.
cAMP Hunter™ GLP1R Dual Protocols: Suspension & Adherent
The cAMP Hunter™ Human GLP-1 (GLP1R) Gs Stable Cell Line Assay (CHO-K1, cat. no. 795-0062C2) offers dual-protocol flexibility: a traditional adherent mode for overnight cell attachment and standard workflows, and a streamlined suspension mode for same-day results in ~3 hours with reduced hands-on time. Both deliver equivalent potency (e.g., Exendin-4 EC50), high signal-to-background ratios, and seamless EFC-based cAMP detection, validated specifically for this GLP1R line to support HTS, automation, or routine screening.
Typical Results: Suspension vs. Adherent
The following graphs show typical results obtained with the cAMP HitHunter kit using two different workflows, demonstrating robust performance regardless of the workflow chosen.
Typical results obtained on cAMP Hunter GLP1R Gs Cell Line (CHO-K1) (#795-0062C2). Exendin-4 dose-response curves generated using cAMP HitHunter kit in adherent mode (A.) and suspension mode (B.).