cAMP Hunter™ Human GLP-1 (GLP-1 Receptor) Gs Cell-Based Assay Kit (HEK 293)

Background Information:

The cAMP Hunter™ Human GLP-1 (GLP-1 Receptor) Gs Cell-Based Assay Kit (HEK 293) utilizes the Human GLP1R cAMP cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of cAMP signaling following receptor activation for drug discovery and development programs. This cell line enables assessment of ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This assay utilizes EFC technology to measure ligand-induced activation by quantifying intracellular cAMP levels. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This product leverages a sensitive chemiluminescent cAMP detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Coupling:

Gs

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062.3

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

HEK293