cAMP Hunter™ eXpress OPRM1 CHO-K1 GPCR Assay

Background Information:

The cAMP Hunter™ OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met-enkephalin, morphine) based activation of OPRM1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRM1, a naturally Gi/Go coupled GPCR. Upon activation of OPRM1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Opioid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

mu (MOP)

Protein Aliases:

MOR-1|Mu opiate receptor|Mu opioid receptor|MOP|hMOP

Accession Number:

NM_000914.3

UniProt Number:

P35372

Gene Name:

OPRM1

Gene ID:

4988

Gene Aliases:

MOR1

Target Species:

Human

Cell Background:

CHO-K1