cAMP Hunter™ eXpress Human GLP1R (Low Expression) Gs Assay (CHO-K1)

Background Information:

The cAMP Hunter™ eXpress Human GLP‑1R (Low Expression) kit provides an easy-to-use cell-based assay to study drug candidates (small molecules, peptides, or biologics) targeting the glucagon-like peptide-1 receptor (GLP‑1R). This kit assesses ligand-induced GLP‑1R activation through the detection of intracellular cAMP accumulation using EFC technology. Unlike conventional overexpression systems, the cells express lower levels of wild-type human GLP‑1R, providing receptor densities that are closer to physiological conditions. This more biologically relevant model can help reduce receptor reserve effects and improve the ability to distinguish differences in ligand potency and efficacy, supporting more predictive pharmacological characterization of GLP‑1R agonists. Upon receptor activation, increased intracellular cAMP levels generate an amplified EFC signal when the cAMP detection reagents are applied.
eXpress kits are a convenient, ready-to-use format that includes all materials required to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Coupling:

Gs

Protein Name:

GLP-1

Protein Aliases:

GLP-1 receptor|GLP-1-R|GLP-1R

Accession Number:

NM_002062.3

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Gene Aliases:

GLP-1R|GLP-1-R

Target Species:

Human

Cell Background:

CHO-K1