cAMP Hunter™ eXpress CXCR5 (BLR1) CHO-K1 GPCR Assay

Background Information:

The cAMP Hunter™ CXCR5 (BLR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL13/BCA-1/BLC) based activation of CXCR5 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR5, a naturally Gi/Go coupled GPCR. Upon activation of CXCR5, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR5

Protein Aliases:

CXCR-5|Burkitt lymphoma receptor 1|Monocyte-derived receptor 15|MDR-15|CD antigen CD185

Accession Number:

NM_001716

UniProt Number:

P32302

Gene Name:

CXCR5

Gene ID:

643

Gene Aliases:

MDR15|CD185

Target Species:

Human

Cell Background:

CHO-K1