cAMP Hunter™ eXpress CALCRL-RAMP1 CHO-K1 GPCR Assay

Background Information:

The cAMP Hunter™ CALCRL-RAMP1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. β-CGRP) based activation of CALCRL (paired with RAMP1) GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCRL, a naturally Gs coupled GPCR, alongside modulator protein RAMP1. Upon activation of CALCRL, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Calcitonin

Sub Family:

Class B

Coupling:

Gs

Protein Name:

CLR

Protein Aliases:

Calcitonin receptor-like receptor

Accession Number:

AY389506

UniProt Number:

Q16602

Gene Name:

CALCRL

Gene ID:

10203

Gene Aliases:

CGRPR|CRLR

Target Species:

Human

Cell Background:

CHO-K1