cAMP Hunter™ eXpress PTGIR CHO-K1 GPCR Assay

Background Information:

The cAMP Hunter™ PTGIR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin I2, beraprost) based activation of PTGIR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTGIR, a naturally Gs coupled GPCR. Upon activation of PTGIR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Coupling:

Gs

Protein Name:

IP

Protein Aliases:

PGI receptor|PGI2 receptor|Prostanoid IP receptor

Accession Number:

AY242134

UniProt Number:

P43119

Gene Name:

PTGIR

Gene ID:

5739

Gene Aliases:

IP|PRIPR

Target Species:

Human

Cell Background:

CHO-K1