Biological Information
Background Information:
The cAMP Hunter™ VIPR2 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type VIPR2 , a naturally Gs coupled GPCR. Upon activation of VIPR2 , the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
VIP and PACAP
Sub Family:
Class B
Coupling:
Gq & Gs
Protein Name:
VIP2 (VPAC2)
Protein Aliases:
VIP and PACAP receptor 2
Accession Number:
NM_003382
UniProt Number:
P41587
Gene Name:
VIPR2
Gene ID:
7434
Gene Aliases:
VPAC2|VPAC2R
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
cAMP Detection
Detection Method:
Chemiluminescence
Additional Information
Brand:
cAMP Hunter™