Biological Information
Background Information:
The cAMP Hunter™ ADORA1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. adenosine, NECA) based activation of ADORA1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADORA1 , a naturally Gi/Go coupled GPCR. Upon activation of ADORA1 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Adenosine
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
A1
Accession Number:
AY136746
UniProt Number:
P30542
Gene Name:
ADORA1
Gene ID:
134
Gene Aliases:
RDC7
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
cAMP Detection
Detection Method:
Chemiluminescence
Additional Information
Brand:
cAMP Hunter™