ADORA2A (A2A) Gq Calcium Signaling Cell-Based Assay Kit (RBL-2H3)

Background Information:

The ADORA2A (A2A) Gq Calcium Signaling Cell-Based Assay Kit (RBL-2H3) utilizes the ADORA2A Gq cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) that target GPCRs. This cell line enables the assessment of ligand (e.g., adenosine, NECA) based activation of ADORA2A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADORA2A, a naturally Gq coupled GPCR. Upon activation of ADORA2A, the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Adenosine

Sub Family:

Class A

Coupling:

Gq & Gs

Protein Name:

A2A

Accession Number:

NM_000675.4

UniProt Number:

P29274

Gene Name:

ADORA2A

Gene ID:

135

Gene Aliases:

RDC8|ADORA2

Target Species:

Human

Cell Background:

RBL-2H3