SPRINTer™ STAT3 Protein Turnover Biosensor Cell Line (A549)

Background Information:

SPRINTer™ Protein Turnover Biosensor cell lines are engineered to introduce the EFC Enzyme Donor (ED) into the endogenous locus of the desired target gene. Expression of the target gene from its native promoter results in production of an ED-tagged target protein. Treatment of the engineered Biosensor cells with therapeutics that promote turnover of the target protein produces a decrease in EFC signal. Addition of exogenous Enzyme Acceptor (EA) and buffer lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal, allowing quantitation of drug-induced changes in ED-target protein levels.

Protein Name:

STAT3

Accession Number:

NM_001369512

UniProt Number:

P40763

Gene Name:

STAT3

Gene ID:

6774

Gene Aliases:

APRF

Target Species:

Human

Cell Background:

A549