Ready-to-Assay beta2 Adrenergic Receptor Frozen Cells

Background Information:

The Ready-to-Assay β2 Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRB2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADRB2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRB2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Protein Name:

beta2

Protein Aliases:

Beta-2 adrenoceptor

Accession Number:

NM_000024

UniProt Number:

P07550

Gene Name:

ADRB2

Gene ID:

154

Gene Aliases:

ADRBR|BAR|B2AR

Target Species:

Human

Cell Background:

Chem-1