PathHunter® eXpress rGPR120 CHO-K1 β-Arrestin GPCR Assay

Background Information:

The PathHunter® rGPR120 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. free fatty acids) based activation of rGPR120 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged rGPR120 and EA-tagged β-Arrestin-2. Activation of rGPR120 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein.

Target Class:

GPCR

Family:

Free Fatty Acid

Sub Family:

Class A

Coupling:

Gq

Protein Name:

FFA4 (GPR120)

Protein Aliases:

G-protein coupled receptor 129|G-protein coupled receptor GT01|G-protein coupled receptor PGR4|Omega-3 fatty acid receptor 1

Accession Number:

NM_001047088

UniProt Number:

Q2AC31

Gene Name:

FFAR4

Gene ID:

294075

Gene Aliases:

PGR4

Target Species:

Rat

Cell Background:

CHO-K1