PathHunter® eXpress mEDG1 CHO-K1 β-Arrestin GPCR Assay

Background Information:

The PathHunter® mEDG1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. S1P) based activation of mEDG1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged mEDG1 and EA-tagged β-Arrestin-2. Activation of mEDG1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

S1P1

Protein Aliases:

EDG1|Endothelial differentiation G-protein coupled receptor 1|Sphingosine 1-phosphate receptor Edg-1|S1P receptor Edg-1|CD antigen CD363

Accession Number:

NM_007901

UniProt Number:

O08530

Gene Name:

S1PR1

Gene ID:

13609

Gene Aliases:

edg-1|D1S3362|CD363

Target Species:

Mouse

Cell Background:

CHO-K1