Biological Information

Background Information:

The PathHunter® PTHR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. PTH) based internalization of PTHR2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged PTHR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTHR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Parathyroid Hormone

Sub Family:

Class B

Coupling:

Gq & Gs

Protein Name:

PTH2

Accession Number:

AY497546

UniProt Number:

P49190

Gene Name:

PTH2R

Gene ID:

5746

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

eXpress Assay Kits

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover

Assay Information

Assay Type:

Cell Based

Assay Measures:

Internalization

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®