PathHunter® eXpress KISS1R U2OS β-Arrestin GPCR Assay

Background Information:

The PathHunter® KISS1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. kisspeptin) based activation of KISS1R GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged KISS1R and EA-tagged β-Arrestin-2. Activation of KISS1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Kisspeptin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

KiSS1 (GPR54)

Protein Aliases:

G-protein coupled receptor 54|G-protein coupled receptor OT7T175|hOT7T175|Hypogonadotropin-1|Kisspeptins receptor|Metastin receptor

Accession Number:

NM_032551

UniProt Number:

Q969F8

Gene Name:

KISS1R

Gene ID:

84634

Gene Aliases:

HOT7T175|AXOR12|GPR54

Target Species:

Human

Cell Background:

U2OS