PathHunter® eXpress GPR30 CHO-K1 β-Arrestin Orphan GPCR Assay

Background Information:

The PathHunter® GPR30 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR30 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged GPR30 and EA-tagged β-Arrestin-2. Activation of GPR30 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Orphan receptor with unknown ligand.

Target Class:

GPCR

Family:

G protein-coupled Estrogen

Sub Family:

Class A

Coupling:

Gi/Go & Gs

Protein Name:

GPER (GPR30)

Protein Aliases:

Flow-induced endothelial G-protein coupled receptor 1|FEG-1|G protein-coupled estrogen receptor 1|G-protein coupled receptor 30|GPCR-Br|IL8-related receptor DRY12|Lymphocyte-derived G-protein coupled receptor|LYGPR|Membrane estrogen receptor|mER

Accession Number:

NM_001505

UniProt Number:

Q99527

Gene Name:

GPER1

Gene ID:

2852

Gene Aliases:

CEPR|CMKRL2|DRY12|GPER|GPR30

Target Species:

Human

Cell Background:

CHO-K1