PathHunter® eXpress EphB3 Functional Assay

Background Information:

The PathHunter® eXpress EphB3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged EphB3 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

EphB3

Protein Aliases:

EPH-like tyrosine kinase 2|EPH-like kinase 2|Embryonic kinase 2|EK2|hEK2|Tyrosine-protein kinase TYRO6

Accession Number:

NM_004443.3

UniProt Number:

P54753

Gene Name:

EPHB3

Gene ID:

2049

Gene Aliases:

Hek2|Tyro6

Target Species:

Human

Cell Background:

U2OS