PathHunter® eXpress EphB2 Functional Assay

Background Information:

The PathHunter® eXpress EphB2 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB2 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged EphB2 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

EphB2

Protein Aliases:

Developmentally-regulated Eph-related tyrosine kinase|ELK-related tyrosine kinase|EPH tyrosine kinase 3|EPH-like kinase 5|EK5|hEK5|Renal carcinoma antigen NY-REN-47|Tyrosine-protein kinase TYRO5|Tyrosine-protein kinase receptor EPH-3 [Cleaved into: EphB2/CTF1; EphB2/CTF2]

Accession Number:

NM_017449.3

UniProt Number:

P29323

Gene Name:

EPHB2

Gene ID:

2048

Gene Aliases:

Hek5|Tyro5

Target Species:

Human

Cell Background:

U2OS