Biological Information

Background Information:

The PathHunter® rHTR2C cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of rHTR2C GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged rHTR2C and EA-tagged β-Arrestin 2. Activation of rHTR2C stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rat version of this protein.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

5-HT2C

Protein Aliases:

5-HT2C|5-HTR2C|5-hydroxytryptamine receptor 1C|5-HT-1C|5-HT1C|Serotonin receptor 2C

Accession Number:

NM_012765

UniProt Number:

P08909

Gene Name:

HTR2C

Gene ID:

25187

Gene Aliases:

5ht1c|Htr1c

Target Species:

Rat

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®