PathHunter® U2OS ErbB2-ErbB3 Functional Assay

Background Information:

The PathHunter® U2OS ErbB2-ErbB3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated ErbB2 and ErbB3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged ErbB2 and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, ErbB3 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

HER2

Protein Aliases:

neuro/glioblastoma derived oncogene homolog|human epidermal growth factor receptor 2

Accession Number:

NM_004448.2; NM_001982.2

UniProt Number:

P04626

Gene Name:

ERBB2

Gene ID:

2064

Gene Aliases:

NEU|HER-2|CD340|HER2

Target Species:

Human

Cell Background:

U2OS