PathHunter® TACR3 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® TACR3 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. neurokinins, senkitide, substance P) based internalization of TACR3 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged TACR3. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting TACR3-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Tachykinin

Sub Family:

Class A

Coupling:

Gq & Gs

Protein Name:

NK3

Protein Aliases:

neurokinin beta receptor

Accession Number:

NM_001059

UniProt Number:

P29371

Gene Name:

TACR3

Gene ID:

6870

Gene Aliases:

NK3R|TAC3R

Target Species:

Human

Cell Background:

U2OS