PathHunter® TACR1 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® TACR1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. tachykinin, substance P) based internalization of TACR1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged TACR1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting TACR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Tachykinin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

NK1

Protein Aliases:

NK-1 receptor|NK-1R|Tachykinin receptor 1

Accession Number:

NM_001058

UniProt Number:

P25103

Gene Name:

TACR1

Gene ID:

6869

Gene Aliases:

SPR|NK1R|NKIR|TAC1R

Target Species:

Human

Cell Background:

U2OS