PathHunter® OXER1 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® OXER1 Total GPCR Internalization Cell Line is a stable, engineered cell line designed to study drug candidates (small molecules or biologics) that affect GPCR activation and internalization. This cell line enables the assessment of ligand-induced internalization of OXER1, for example, in response to 5-oxo-ETE. The assay is based on Enzyme Fragment Complementation (EFC) technology. The cell line overexpresses a PK-tagged OXER1 receptor and an Enzyme Acceptor (EA) tag localized to the plasma membrane, enabling complementation of the two β-galactosidase enzyme fragments (EA and PK). Upon ligand-induced receptor activation, OXER1 undergoes internalization, disrupting EA/PK complementation and resulting in a loss of chemiluminescent signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Leukotriene

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

OXE

Protein Aliases:

G-protein coupled receptor 170|G-protein coupled receptor R527|G-protein coupled receptor TG1019

Accession Number:

NM_148962.4

UniProt Number:

Q8TDS5

Gene Name:

OXER1

Gene ID:

165140

Gene Aliases:

GPCR|TG1019|GPR170

Target Species:

Human

Cell Background:

U2OS