Biological Information

Background Information:

The PathHunter® rCHRM4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged rCHRM4 and EA-tagged β-Arrestin 2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rat version of this protein.

Target Class:

GPCR

Family:

Acetylcholine (Muscarinic)

Sub Family:

Class A

Coupling:

Gs & Gi/Go

Protein Name:

M4

Protein Aliases:

acetylcholine receptor, muscarinic 4

Accession Number:

NM_031547

UniProt Number:

P08485 

Gene Name:

CHRM4

Gene ID:

25111

Target Species:

Rat

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®