Biological Information
Background Information:
The PathHunter® mEDG1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. S1P) based activation of mEDG1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged mEDG1 and EA-tagged β-Arrestin 2. Activation of mEDG1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein.
Target Class:
GPCR
Family:
Lysophospholipid (S1P)
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
S1P1
Protein Aliases:
EDG1|Endothelial differentiation G-protein coupled receptor 1|Sphingosine 1-phosphate receptor Edg-1|S1P receptor Edg-1|CD antigen CD363
Accession Number:
NM_007901
UniProt Number:
O08530
Gene Name:
S1PR1
Gene ID:
13609
Gene Aliases:
edg-1|D1S3362|CD363
Target Species:
Mouse
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®