Biological Information
Background Information:
The PathHunter® MTNR1A cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. melatonin) based activation of MTNR1A GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged MTNR1A and EA-tagged β-Arrestin 2. Activation of MTNR1A stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Melatonin
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
MT1
Protein Aliases:
Mel1a receptor
Accession Number:
NM_005958
UniProt Number:
P48039
Gene Name:
MTNR1A
Gene ID:
4543
Gene Aliases:
MEL-1A-R
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®